Microbiological quality of fresh and heat treated cow's milk during storage

The aim of the present study was to evaluate the microbiological quality of raw milk from milk vending machine and heat treated milk during storage. There were analyzed 120 samples of milk (30 samples of fresh milk, 30 samples of raw milk stored 4 day at 4 °C, 30 samples of heat treated milk - 70 °C stored 4 day at 4 °C and 30 samples of heat treated milk - 100 °C stored 4 day at 4 °C). Total viable counts (TVC), coliform bacteria (CB) and microscopic filamentous fungi (MFF) were determined by microbiological analysis. Plate dilution method were used for microbiological analysis. The number of total viable counts (TVC) in fresh milk ranged from 4.08 log KTJ.mL-1 to 4.89 CFU.mL-1. TVC in raw milk after storage ranged from 5.31 log CFU.mL-1 to 6.81 log CFU.mL-1. TVC in heat treated milk with temperature 70 °C after storage ranged from 3.89 log CFU.mL-1 to 4.45 log CFU.mL-1 and TVC in heat treated milk with temperature 100 °C after storage ranged from 2.96 log KTJ.mL-1 to 3.91 log KTJ.mL-1 in heat treated milk with temperature 100 °C after storage. The number of CB were in range from 1.49 log CFU.mL-1 to 1.89 log CFU.mL-1 in fresh milk, from 1.99 log CFU.mL-1 to 2.61 log CFU.mL-1 in raw stored milk. Coliform bacteria were not present in heat-treated milk samples. The values of MFF ranged from 0 log CFU.mL-1 to 2.01 log CFU.mL-1 in fresh milk, from 1.43 log CFU.mL-1 to 3.98 log CFU.mL-1 in raw milk after storage, from 1.33 log CFU.mL-1 to 3.41 log CFU.mL-1 in heat treated milk with temperature 70 °C after storage and from 1.30 log CFU.mL-1 to 3.32 log CFU.mL-1 in heat treated milk with temperature 100 °C after storage.