Determination of flavonoids in extract of ginkgo biloba leaves by high performance liquid chromatography(HPLC)
Jing Wang, Zou Yujia, Huacheng Tang, Dong Li
Abstract
Jing Wang, Zou Yujia, Huacheng Tang, Dong Li
Abstract
用高效液相色谱法测定银杏制品经酸水解后3种黄酮甙元槲皮素、山奈素、异鼠李素的含量,计算得出总黄酮含量,并对6个样品进行了测定.方法采用C18色谱柱,流动相为甲醇:水:磷酸,检测波长370nm,槲皮素、山奈素和异鼠李素的平均回收率分别是95.80%、98.42%和94.39%,RSD≤4.2%,相关系数γ=0.9999,本法准确、快速、重现性好,为银杏制品提供质量控制依据.
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用高效液相色谱法测定银杏制品经酸水解后3种黄酮甙元槲皮素、山奈素、异鼠李素的含量,计算得出总黄酮含量,并对6个样品进行了测定.方法采用C18色谱柱,流动相为甲醇:水:磷酸,检测波长370nm,槲皮素、山奈素和异鼠李素的平均回收率分别是95.80%、98.42%和94.39%,RSD≤4.2%,相关系数γ=0.9999,本法准确、快速、重现性好,为银杏制品提供质量控制依据.
Key concepts: Ginkgo biloba, High-performance liquid chromatography, Chromatography, Chemistry, Ginkgo, Ginkgoales, Traditional medicine, Pharmacognosy