2006•Science and Technology of Food IndustryRequires access

Determination of flavonoids in extract of ginkgo biloba leaves by high performance liquid chromatography(HPLC)

Jing Wang, Zou Yujia, Huacheng Tang, Dong Li

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Abstract

用高效液相色谱法测定银杏制品经酸水解后3种黄酮甙元槲皮素、山奈素、异鼠李素的含量,计算得出总黄酮含量,并对6个样品进行了测定.方法采用C18色谱柱,流动相为甲醇:水:磷酸,检测波长370nm,槲皮素、山奈素和异鼠李素的平均回收率分别是95.80%、98.42%和94.39%,RSD≤4.2%,相关系数γ=0.9999,本法准确、快速、重现性好,为银杏制品提供质量控制依据.

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用高效液相色谱法测定银杏制品经酸水解后3种黄酮甙元槲皮素、山奈素、异鼠李素的含量,计算得出总黄酮含量,并对6个样品进行了测定.方法采用C18色谱柱,流动相为甲醇:水:磷酸,检测波长370nm,槲皮素、山奈素和异鼠李素的平均回收率分别是95.80%、98.42%和94.39%,RSD≤4.2%,相关系数γ=0.9999,本法准确、快速、重现性好,为银杏制品提供质量控制依据.

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Available abstract

用高效液相色谱法测定银杏制品经酸水解后3种黄酮甙元槲皮素、山奈素、异鼠李素的含量,计算得出总黄酮含量,并对6个样品进行了测定.方法采用C18色谱柱,流动相为甲醇:水:磷酸,检测波长370nm,槲皮素、山奈素和异鼠李素的平均回收率分别是95.80%、98.42%和94.39%,RSD≤4.2%,相关系数γ=0.9999,本法准确、快速、重现性好,为银杏制品提供质量控制依据.

Key concepts: Ginkgo biloba, High-performance liquid chromatography, Chromatography, Chemistry, Ginkgo, Ginkgoales, Traditional medicine, Pharmacognosy

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