An Optilal Method for Determination of Main Flavonoids and Terpenoids in Ginkgo Biloba Extract by HPLC

Objective The optimal method ( high performance liquid chromatography, HPLC ) for determination of 3 kinds of flavonoids and 4 kinds of terpenoids in ginkgo biloba extract has been established and validated. Methods Ginkgo biloba extract was hydrolyzed and diluted to determine flavonoids using HPLC. On the other hand, the dissolved ginkgo biloba extract with methanol was cleaned up via an extraction tube packed with acidic aluminum oxide and then used to determine terpenoids also using HPLC determined. Results The linear ranges of quercetin, kaempherol and isorahmnetin were 5 - 60 ng, 5 - 60 ng and 2. 5 -40 ng,respectively. Their average recovery rates was 95.4% and the RSD was 2.3% (re =6). The linear ranges of ginkgolide A, ginkgolide B, ginkgolide C and bilobalide were 0.5 - 7.5 μg. The average recovery rates were 97. 2% ( RSD = 2. 4% ) , 99. 3% ( RSD = 1. 9% ) , 97. 4% ( RSD = 1. 8% ) and 97. 9% ( RSD = 2.5%), respectively. Conclusion The method was simple, rapid and accurate. It can be applied in quantitative determination of flavonoids and terpenoids in ginkgo biloba extract.