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2007•Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary MedicineOpen access

DETECTION OF BORDETELLA BRONCHISEPTICA INFECTION IN SWINE USING AN AGGLUTINATION TEST

Attila Dénes, Gh. Răpuntean

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Abstract

Antibody detection in growing an mature swine naturally infected with Bordetella bronchiseptica was performed using an agglutination test. The antigen was represented of formalin-killed phaseI Bordetella bronchiseptica cells. Under optimum antigen concentration and reaction temperature 63 (42%) of 150 serum samples tested were positive of Bordetella infection. Using this test, antibodyes produced against Pasteurella multocida of growing and mature pigs sera, can cross-react with Bordetella bronchiseptica antigen. Because of this, only agglutinin reactions in 1:20 dilutions or higher to Bordetella were considered positive. The agglutination test appears to be more reliable than isolation of Bordetella bronchiseptica for the identification of carrier state of Bordetella bronchiseptica in swine.

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Antibody detection in growing an mature swine naturally infected with Bordetella bronchiseptica was performed using an agglutination test. The antigen was represented of formalin-killed phaseI Bordetella bronchiseptica cells. Under optimum antigen concentration and reaction temperature 63 (42%) of 150 serum samples tested were positive of Bordetella infection. Using this test, antibodyes produced against Pasteurella multocida of growing and mature pigs sera, can cross-react with Bordetella bronchiseptica antigen. Because of this, only agglutinin reactions in 1:20 dilutions or higher to Bordetella were considered positive. The agglutination test appears to be more reliable than isolation of Bordetella bronchiseptica for the identification of carrier state of Bordetella bronchiseptica in swine.

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Available abstract

Antibody detection in growing an mature swine naturally infected with Bordetella bronchiseptica was performed using an agglutination test. The antigen was represented of formalin-killed phaseI Bordetella bronchiseptica cells. Under optimum antigen concentration and reaction temperature 63 (42%) of 150 serum samples tested were positive of Bordetella infection. Using this test, antibodyes produced against Pasteurella multocida of growing and mature pigs sera, can cross-react with Bordetella bronchiseptica antigen. Because of this, only agglutinin reactions in 1:20 dilutions or higher to Bordetella were considered positive. The agglutination test appears to be more reliable than isolation of Bordetella bronchiseptica for the identification of carrier state of Bordetella bronchiseptica in swine.

Key concepts: Bordetella bronchiseptica, Bordetella, Pasteurella multocida, Microbiology, Bordetella pertussis, Direct agglutination test, Agglutination (biology), Biology

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