2016•PubMedOpen access

[Construction of the pIRES2-ZsGreen1 eukaryotic expression vector of Factor Ⅸ gene and expression in HEK-293 cells].

J F Chen, Y F Zhang, Joo‐Hyun Kang, Xiaolin Qin, M F Wang, G Wang, L H Yang

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Abstract

The experimental results showed the expression vector, pIRES2-ZsGreen1/FⅨ, was successfully constructed , which provided experiment basement for the follow study on the location, function and molecular pathology of hemophilia B.

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What this paper is about

The experimental results showed the expression vector, pIRES2-ZsGreen1/FⅨ, was successfully constructed , which provided experiment basement for the follow study on the location, function and molecular pathology of hemophilia B.

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Available abstract

The experimental results showed the expression vector, pIRES2-ZsGreen1/FⅨ, was successfully constructed , which provided experiment basement for the follow study on the location, function and molecular pathology of hemophilia B.

Key concepts: Transfection, HEK 293 cells, Molecular biology, Expression vector, Complementary DNA, Plasmid, Biology, Gene expression

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[Construction of the pIRES2-ZsGreen1 eukaryotic expression vector of Factor Ⅸ gene and expression in HEK-293 cells]. — Research Paper | ScholarLens