Isolation, characterization and identification of bacterial strain showing antimicrobial activity

Natural sources such as plants, microorganisms and animals provide a range of natural medicinal mixtures for the treatment of various infectious diseases. There is increasing need to search new antibacterial agents due to the rising incidences of bacterial infections and their resistance towards antibiotics.The antimicrobial production is a common phenomenon for most bacteria. Due to this reason the aim of the present study was focused on the isolation, of microbial strains showing antimicrobial activity from the soil collected near the roots of Calotropis species growing at UIET campus of M D University Rohtak. Microorganisms were isolated by serial dilution, plating method and grown on nutrient agar medium. All isolated strains were tested for antimicrobial activity using agar well diffusion method. Out of 8 isolates 7 strains showed antimicrobial activity. The characterization of isolates was carried out by morphological method, biochemical method and molecular characterization techniques. The isolate having highest antimicrobial activity was identified as Bacillus cereus by16S rRNA sequence analysis.