Establishment and application of a double RT-PCR for classical swine fever virus and porcine reproductive and respiratory syndrome virus.

According to the nucleotide sequences of classical swine fever virus(CSFV)and porcine reproductive and respiratory syndrome virus(PRRSV)in GenBank,two pairs of primers were designed and synthesized.A double RT-PCR was developed based on these two pairs of primers which amplified the CSFV virus-specific segment with 508bp and the PRRSV virus-specific segment with 432bp in sizes after the conditions of PCR were optimized.The results indicate that the double RT-PCR reaction after optimization show a good sensitive and specific,repeatable,stability,simple,rapid and highly effect for diagnosis CSFV and PRRSV.It can be used in rapid diagnosis of CSFV and PRRSV in further epidemiological investigation.