Establishment of Duplex RT-PCR Method for Detecting Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus

In order to establish a rapid,differential diagnosis method for classical swine fever virus(SCFV)and porcine reproductive and respiratory syndrome virus(PRRSV),we designed a pairs of specfic primers targeting to the conservative genes according to the genomic sequence of CSFV and PRRSV in GenBank,and established a duplex RT-PCR method for simultaneously detecting SCFV and PRRSV.This method can amplify 310 bp and/or 161 bp specific fragments from either SVFV and PRRSV single sample or mixed samples,and the minimum RNA content for detection is 0.2 pg.This methed could specifically detect SCFV and PRRSV,while amplification results for JEV,PEDV,PGEV,PPV,PCV-2,PRV were negative.The differential diagnosis RT-PCR method in this study had good sensitivity,specificity and repeatability.It can accurately,quickly,and differentialy diagnose CSFV and PRRSV in a PCR system,which can be used for rapid detection of clinical samples as well.