Biofilm formation by Multidrug resistant Enterococcus faecalis (MDEF) originated from clinical samples

Enterococci, leading causes of nosocomial bacteremia, surgical wound infection, and urinary tract infection, are becoming resistant to all standard therapies. The present study is to monitore the Enterococcus faecalis strains to form biofilms screened by tissue culture plate (TCP), Tube method (TM). Biofilm formation potential was determined by growing the tested strains containing BHI medium. The capability of forming biofilms was detected in 40 MDR resistant E.faecalis strains, strong biofilm producers were 11(27.5%), 22(55%) were moderate and 07(17.5%) isolates were considered as non or weak biofilm producers. Though TM correlated well with TCP test for 09 (22.5%), 16(40.0%) and 15(37.5%) strongly biofilm producing strains, weak producers were difficult to discriminate from bioflim negative isolates. The present study shows a significant difference in production of biofilm, therefore, it can be concluded that biofilm production has an important role in causing nosocomial infection. Keywords: Enterococcus, E. faecalis, MDR, Bioflim.