Biofilm formation among enterococci species

Enterococci are an important member of human gastrointestinal flora, and are also an important cause of opportunistic nosocomial infections. The ability of enterococci to form biofilms may confer an ecological advantage in certain situations. For example, clinical strains of E. faecalis isolated from infective endocarditis patients were significantly associated with the greater biofilm formation than nonendocarditis clinical isolates. A sum of 20 clinical isolates of Enterococcus spp were subjected to antibiotic susceptibility pattern followed by detection of biofilm formation by TCP method. We found varied resistance pattern by disc diffusion method and none of the isolates was found to be vancomycin resistance. We have very little percentage of biofilm formers this indicates the less prevalence of biofilm in enterococci in our isolates. However in order to detect the same, more number of isolates to be included from different specimens. This TCP method is a much reliable and convenient method to detect the biofilm load in even a simple lab setup.