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2015•Unpublished venueRequires access

MODERN METHODS FOR DETERMINATION OF BINDING CONSTANTS:CAPILLARY ELECTROPHORESIS VS. ISOTHERMAL TITRATION CALORIMETRY

Hana Nevídalová, Zdeněk Glatz, Lenka Michalcová

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Abstract

Strength of the binding between ligands (drugs) and proteins is commonly described by binding constant (Kb). These interactions have a significant effect on the biological activity, pharmacodynamics and pharmacokinetics properties of drugs. Kb can be determined by several methods. In this study capillary electrophoresis-frontal analysis (CE-FA) and isothermal titration calorimetry (ITC) are compared. Kb of systems diclofenac-HSA and lidocaine-HSA were measured by both methodologies. The results of CE-FA and ITC are comparable and can be used for investigation of other binding parameters.

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What this paper is about

Strength of the binding between ligands (drugs) and proteins is commonly described by binding constant (Kb). These interactions have a significant effect on the biological activity, pharmacodynamics and pharmacokinetics properties of drugs. Kb can be determined by several methods. In this study capillary electrophoresis-frontal analysis (CE-FA) and isothermal titration calorimetry (ITC) are compared. Kb of systems diclofenac-HSA and lidocaine-HSA were measured by both methodologies. The results of CE-FA and ITC are comparable and can be used for investigation of other binding parameters.

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Available abstract

Strength of the binding between ligands (drugs) and proteins is commonly described by binding constant (Kb). These interactions have a significant effect on the biological activity, pharmacodynamics and pharmacokinetics properties of drugs. Kb can be determined by several methods. In this study capillary electrophoresis-frontal analysis (CE-FA) and isothermal titration calorimetry (ITC) are compared. Kb of systems diclofenac-HSA and lidocaine-HSA were measured by both methodologies. The results of CE-FA and ITC are comparable and can be used for investigation of other binding parameters.

Key concepts: Isothermal titration calorimetry, Capillary electrophoresis, Chemistry, Titration, Binding constant, Isothermal process, Chromatography, Electrophoresis

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MODERN METHODS FOR DETERMINATION OF BINDING CONSTANTS:CAPILLARY ELECTROPHORESIS VS. ISOTHERMAL TITRATION CALORIMETRY — Research Paper | ScholarLens