2015•Unpublished venueRequires access
Comparison of capillary electrophoresis and isothermal titration calorimetry for determination of the binding constant
Lenka Michalcová, Hana Nevídalová, Zdeněk Glatz
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Abstract
The binding constant is commonly used to describe the strength
of binding between ligands (drugs) and protein. The strength of
the interaction has a significant effect on the biological
activity of the drug, and knowledge of the nature and extent of
drug-protein binding can help us to understand the
pharmacokinetics and pharmacodynamics of a drug. The binding
constant may be determined by various methods. The main
objective of the study was to compare the results of capillary
electrophoresis and isotermal titration calorimetry.