2016•Zhonghua neifenmi daixie zazhiRequires access
The effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1
Jun Li, Shuang Jin, Juan Zhang, Wei Zhang, Haofeng Zhou, Huijuan Zhang
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Abstract
Objective
To investigate the effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1 exposed to higher glucose concentration.
Methods
MC3T3-E1 cells were cultured and divided into control group and liraglutide group. In liraglutide group, cells were treated with different liraglutide concentrations (10-9 mol/L, 10-8 mol/L, and 10-7 mol/L, respectively) for 48 hours. Cell proliferation was tested with CCK-8. The mRNA expressions of type Ⅰ collagen (COL-Ⅰ), osteopontin (OPN), and alkaline phosphase (ALP) were detected using semi-quantitative RT-PCR.
Results
(1)Compared to control group, the proliferation rate of different liraglutide concentration groups (10-9 mol/L, 10-8 mol/L, and 10-7 mol/L) increased significantly (all P<0.05), the proliferation rate was the highest in 10-8mol/L liraglutide group. (2)The expression of COL-Ⅰ, OPN, and ALP mRNA in liraglutide groups were higher than those in control group (all P<0.05). The optimal concentration of liraglutide was 10-8 mol/L.
Conclusion
Liraglutide within a certain concentration range may improve the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1. (Chin J Endocrinol Metab, 2016, 32: 232-235)
Key words:
Glucagon- like peptide-1 analogue; Liraglutide; Osteoblast; Proliferation; Differentiation