The effect of different concentration of zinc sulfate on the gene expression of osteoprotegerin and cell proliferation of MC3T3-E1 osteoblasts

Objective To explore the effect of different concentration of zinc on the gene expression of osteoprotegerin and cell proliferation of osteoblasts.Methods The mouse osteoblast cell line MC3T3-E1 were cultured and randomly divided into 4 groups.Sulfate zinc was added into Group A(10 μmol/L),Group B(50 μmol/L),and Group C(200 μmol/L).Group D was treated as control group.RNA was extracted from cells after 48-hour treatment.The mRNA expression of osteoprotegerin was analyzed using RT-PCR method.The expression of osteoprotegerin in cell culture medium was measured using Elisa method.The proliferation rate of osteoblasts was measured using MTT method.Results The mRNA expressions of osteoprotegerin in group A,B,C,and D were 0.461±0.051,1.068±0.123,0.244±0.044,and 0.548±0.089,respectively.The difference between group A and D was not statistically significant.The expression in Group B was higher than that in group D(P0.01).The expression in Group C was lower than that of group D(P0.01).The Elisa results were identical with those of RT-PCR.The osteoblast proliferation was significantly different.It was higher in Group B than in group D(P0.01),and was lower in Group C than in group D(P0.01).Conclusion Zinc of 50 μmol/L promotes the gene expression of osteoprotegerin and proliferation of osteoblasts.Zinc of 200 μmol/L inhibits the gene expression of osteoprotegerin and proliferation of osteoblasts.There is little effect of 10μmol/L zinc on the gene expression of osteoprotegerin and proliferation of osteoblast.