2016•PubMedOpen access

[Protective effect of dexmedetomidine against glutamate-induced cytotoxicity in PC12 cells and its mechanism].

Zhang Wd, Hao Zhang, H Wang, N Zhang, Du Cy, Jingui Yu, Feng Zg

Abstract

OBJECTIVE: To investigate the protective effects of dexmedetomidine (Dex) against glutamate-induced cytotoxicity in PC12 cells and its mechanism. METHODS: was detected by Fluo-8 staining and flow cytometry, and the mitochondrial membrane potential (MMP) was determined with JC-1 staining and flow cytometry. RESULTS: level (P<0.01) and maintained a stable MMP (P<0.01). CONCLUSION: Dexmedetomidine can protect PC12 cells against glutamate-induced injury possibly in relation with its anti-oxidative activity, inhibitory effect on intracellular calcium overload and protective effect of the mitochondria.

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Key concepts: Flow cytometry, Cytotoxicity, Intracellular, Glutamate receptor, Viability assay, Chemistry, Pharmacology, MTT assay