2008•The Journal of Pediatrics of Korean MedicineRequires access
The Effect of Ikhwang-San on Immune Suppression Induced by Methotrexate in SD Rats
Yu-Boo Park, Hyejin Yun, Jung-Han Baek
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Abstract
AbstractThe Effect of Ikhwang-San on Immune Suppression Induced by Methotrexate in SD Rats Park Yu Boo, Yun Hye Jin, Baek Jung HanDepartment of Pediatrics, College of Oriental Medicine, Daegu H aany University ObjectivesThis study is to investigate how dose Ikhwang-San can be effective on SD rats which deteriorated immunity caused by methotrexate.Methods The test sample were dosed once a day for 14 days by gastric gavage at the beginning of dosage 1000, 500 and 250㎎/㎏/10㎖ from 2 days after last MTX-dosing, and the changes of the body and spleen weight, total number of blood leukocytes , total number of lymphocytes, the percentage of B-cell, T-cell, CD3+CD4+ T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the blood and sp leen were observed. Results The changes of the body and spleen weight, the total number of blood leukocytes, the total number of lymphocyte in the blood and spleen were significantly increased in IHS Extracts groups comparing with the control group. The percentage of B-cell, T-cell, CD3+CD4+ T-cell in the blood and spleen were significantly increase in IHS groups and comparing with the control group. The ratio of CD4+/CD8+ T-cell in blood and spleen was significantly increased in IHS Extracts groups comparing with the control group.ConclusionsAccording to those results, Ikhwang-San has good immunostimulating effect on SD rats which had deteri orated immunity caused by methotrexate.Key words : Ikhwang-San (IHS), methotrexate, CD3+CD4+ T-cell, CD3+CD8+ T-cell, CD4+/CD 8+ T-cell ratios