The changes of T lymphocyte subsets by complex Chinese crude drug “Qinkangsan” on chicken artificially challenged by NDV

Two hundred and fifty avian broilers of 10-day-old were divided into 5 groups randomly.All groups except negative control group were injected NDV-F48E9 at 21-day-old,then the Chinese herbal medicine Qinkangsan was add to basal diet at 22 to 25 days age.The supplement dosages were 0.5%(Ⅰ),1.0%(Ⅱ),2.0%(Ⅲ),0(negative control group,Ⅳ),and 0(positive control group,Ⅴ),respectively.The concentrations of CD3+,CD4+,CD8+,and CD4+/CD8+T lymphocyte subsets in peripheral blood were measured at 20,22,24,28 days old by using the flow cytometry,respectively.The results were shown as following:on 22 days old,the concentrations of CD3+,CD4+,CD8+,and CD4+/CD8+T lymphocyte subsets in peripheral blood in all of the groups(except negative control group)were significantly reduced when compared with those of 20 days old.While on 24 days old,the concentrations of CD3+,CD4+,and CD4+/CD8+T lymphocyte subsets in peripheral blood in all of the treatment groups were significantly increased when compared with those of groupⅤ(P0.01).The results indicated thatQinkangsancould promote the growth of T lymphocytes,and increase the ratio of CD4+/CD8+.Thus,the Chinese herbal medicineQinkangsancould increase the cell immune capability of poultry.