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2000•Fungal Genetics ReportsOpen access

A simple modification of the A. nidulans transformation protocol increases the transformation frequency

J A Cockram, Sealy-Lewis

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Abstract

After transformation of Aspergillus nidulans with plasmid DNA the transformants are usually incubated at 37C until transformants appear. We have found that pre-incubation of the transformation plates at room temperature for 24h leads to increased transformation frequencies.

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What this paper is about

After transformation of Aspergillus nidulans with plasmid DNA the transformants are usually incubated at 37C until transformants appear. We have found that pre-incubation of the transformation plates at room temperature for 24h leads to increased transformation frequencies.

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Available abstract

After transformation of Aspergillus nidulans with plasmid DNA the transformants are usually incubated at 37C until transformants appear. We have found that pre-incubation of the transformation plates at room temperature for 24h leads to increased transformation frequencies.

Key concepts: Transformation (genetics), Aspergillus nidulans, Incubation, Plasmid, Chemistry, Transformation efficiency, DNA, Biology

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