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1995•FEMS Microbiology LettersRequires access

Transformation ofAspergillus nidulansby microprojectile bombardment on intact conidia

Maria Helena Pelegrinelli Fungaro, Elíbio Rech, Gilda Santos Mühlen, Marilene Henning Vainstein, Renata Castiglioni Pascon, Marisa Vieira de Queiroz, Aline Aparecida Pizzirani‐Kleiner, João Lúcio de Azevedo

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Abstract

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.

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What this paper is about

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.

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Available abstract

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.

Key concepts: Aspergillus nidulans, Transformation (genetics), Auxotrophy, Plasmid, Conidium, Biology, DNA, Microbiology

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