2000•Journal of Biomolecular Structure and DynamicsRequires access
Application of Elimination Methods in Electrochemical Study ofDNA
Libuše Trnková, René Kizek, O. Dračka
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Abstract
Adsorptive stripping voltammetry has been extended by
elimination voltammetry with the linear scan rate (EVLS). EVLS
offers the elimination of selected currents from the linear
scan voltammetry results. It can be achieved by an elimination
function formed by a linear combination of total currents
measured at different scan rates. The problem of substance
adsorption influence on the elimination voltammetry results has
been studied. Theoretical curves of an irreversible current
with adsorbed substance being electroactive were calculated for
three elimination functions. As a practical application, the
adsorptive stripping voltammetry of thermally denatured DNA on
a hanging mercury electrode has been developed. While the LSV
signal of single-stranded DNA at low concentrations gives a
slight hint of the cathodic peak, the EVLS provides quite a
clear signal in the form of the peak-counterpeak. It is
possible to determinate DNA at concentrations below of
micrograms per milliliter.