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2014•Encyclopedia of Environmental ChangeRequires access

SECONDARY ION MASS SPECTROMETRY (SIMS)

John A. Matthews

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Abstract

Transmission electron microscopy (TEM)/Secondary ion mass spectrometry (SIMS) biofilm sample preparation: Samples of biofilm were fixed with 4 % glutaraldehyde, as described previously (S1). TEM images (Fig. S4) confirm that, because the sectioning resin neither displaces nor damages finescale biofilm features, ultramicrotomed samples of biofilm used for TEM imaging were also suitable for SIMS analyses.

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What this paper is about

Transmission electron microscopy (TEM)/Secondary ion mass spectrometry (SIMS) biofilm sample preparation: Samples of biofilm were fixed with 4 % glutaraldehyde, as described previously (S1). TEM images (Fig. S4) confirm that, because the sectioning resin neither displaces nor damages finescale biofilm features, ultramicrotomed samples of biofilm used for TEM imaging were also suitable for SIMS analyses.

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Available abstract

Transmission electron microscopy (TEM)/Secondary ion mass spectrometry (SIMS) biofilm sample preparation: Samples of biofilm were fixed with 4 % glutaraldehyde, as described previously (S1). TEM images (Fig. S4) confirm that, because the sectioning resin neither displaces nor damages finescale biofilm features, ultramicrotomed samples of biofilm used for TEM imaging were also suitable for SIMS analyses.

Key concepts: Secondary ion mass spectrometry, Static secondary-ion mass spectrometry, Mass spectrometry, Ion-mobility spectrometry–mass spectrometry, Chemistry, Ion, Analytical Chemistry (journal), Mass spectrometry imaging

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