In Vitro Plant Regeneration from Organ Cultures of Gmelinaarborea Roxb

In vitro regeneration of plantlets was obtained using stem (node, internode) and shoot-tip of Gmelina arborea as explants. Explants were cultured on Murashige and Skoog's (1962) medium containing 0.8% agar and different combinations and concentrations of auxin and cytokinin. Techniques were developed for multiple shoot formation directly from nodal and shoot-tip explants as well as shoot regeneration from the callus. Kinetin (Kn) alone at 9.3 μM was most effective and induced the formation of direct multiple shoots in culture. 10.7 μM NAA(a-naphthalene acetic acid) and 9.3 μM Kn resulted in shoot differentiation from the callus. Callus was greenish-white, compact, hydrated and crystalline in appearance. Rooting of the excised shoots was obtained on medium (1/2MSsalts) fortified with IBA (i ndole-3 butyric acid) and NAA. 2, 4-D (2, 4-dichlorophenoxy acetic acid) was, however, suitable for callus induction, not for regeneration. Regenerants were genetically identical to the parent.