Direct shoot regeneration, callus induction and plant regeneration from callus tissue in Mose Rose (Portulaca grandiflora L.)

This experiment was performed to optimize the medium for tissue culture of Mose Rose. Different tissue culture approaches such as shoot regeneration from nodal segments, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 30 g/l sucrose, 8 g/l agar and different plant growth regulators. Nodal segments of Mose Rose were cultured on media containing different levels of BAP or kinetin (0, 5 and 10 M) to direct shoot regeneration study. Leaf explants were cultured in different combination of BAP (at three levels: 2.5, 5 and 10 M) and NAA (at two levels: 5 and 10 M) to examine callus formation. After the callus formation the formed calli were cultured on different combinations of BAP and NAA for shoot regeneration. BAP at three levels (5 and 10 and 15 M) and NAA at two levels (5 and 10 M) in all possible combinations (totally 6 hormonal treatments) were used for shoot regeneration from callus. The results showed that the treatment containing 10 M BAP was found to be the best one for shoot regeneration from nodal segments. The treatment with 10 M NAA in combination with 10 or 5 M BAP were found to be suitable treatments for callus production from leaf explants, as well. Moreover, 15 M BAP alone or in combination with 5 M NAA were found to be the best treatments for shoot regeneration from callus.