Analysis and amplification of Phytase gene in transgenic wheat by polymerase Chain Reaction

In the present study, we report the conformational analysis of phytase gene containing transgenic wheat plants using polymerase chain reaction. Wheat is one of the most widely grown crops in Pakistan. To improve the use of organic phosphate by wheat, phytase gene was expressed in wheat under the control of root specific promoter. Phytase gene enhances the degradation of phytic acid by the release of phytase. In the present study, transgenic wheat plants containing phytase gene were screened. About 300 plants were selected for basta screening. All of these plants were shifted to pots and kept in green house carefully. DNA extraction was carried out from survived 150 plants. Integration of phytase gene was checked with the help of PCR using gene junction primers of all of these genomic DNA samples. PCR results showed that gene has been successfully integrated in 51 transgenic wheat plants.