2015•Turkish Journal of Pharmaceutical SciencesOpen access

Antioxidant Properties Of Rubus Discolor L. Extracts And Protective Effects Of Its Flower Extract Against Hydrogen Peroxide-Induced Oxidative Stress In Wistar Rats

Serhat Keser, Sait Çeli̇k, Semra Türkoğlu, Ökkeş Yılmaz, İ̇smail Türkoğlu

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Abstract

peroxidation and reducing power) of extracts were determined by spectrophotometric methods and HPLC. The antioxidant potential of RD flower extract supplementations were evaluated by measuring total protein, glutathione (GSH), malondialdehyde (MDA), vitamin A and E, cholesterol, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) levels in various tissues. The flower extracts were the most active extracts for in vitro antioxidant assays. In HP administered groups, MDA level was increased, GSH and total protein levels were decreased. In RD treated group, these substances levels were protected. In RD plus HP treated group, SOD and GSH-Px levels were increased. These results show that RD flower extract have the protective effects against H2O2 via its free radical scavenging activity, and this extract affected the level of antioxidant enzymes, vitamin A and E, cholesterol, GSH, total protein and MDA.

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peroxidation and reducing power) of extracts were determined by spectrophotometric methods and HPLC. The antioxidant potential of RD flower extract supplementations were evaluated by measuring total protein, glutathione (GSH), malondialdehyde (MDA), vitamin A and E, cholesterol, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) levels in various tissues. The flower extracts were the most active extracts for in vitro antioxidant assays. In HP administered groups, MDA level was increased, GSH and total protein levels were decreased. In RD treated group, these substances levels were protected. In RD plus HP treated group, SOD and GSH-Px levels were increased. These results show that RD flower extract have the protective effects against H2O2 via its free radical scavenging activity, and this extract affected the level of antioxidant enzymes, vitamin A and E, cholesterol, GSH, total protein and MDA.

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Available abstract

peroxidation and reducing power) of extracts were determined by spectrophotometric methods and HPLC. The antioxidant potential of RD flower extract supplementations were evaluated by measuring total protein, glutathione (GSH), malondialdehyde (MDA), vitamin A and E, cholesterol, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) levels in various tissues. The flower extracts were the most active extracts for in vitro antioxidant assays. In HP administered groups, MDA level was increased, GSH and total protein levels were decreased. In RD treated group, these substances levels were protected. In RD plus HP treated group, SOD and GSH-Px levels were increased. These results show that RD flower extract have the protective effects against H2O2 via its free radical scavenging activity, and this extract affected the level of antioxidant enzymes, vitamin A and E, cholesterol, GSH, total protein and MDA.

Key concepts: Hydrogen peroxide, Oxidative stress, Antioxidant, Traditional medicine, Chemistry, Biology, Botany, Biochemistry

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