IVITRO ATIOXIDAT ACTIVITY AD PHYTOCHEMICAL SCREEIG OF ETHAOLIC EXTRACT OF Tabernaemontana coronaria (L.)

Summary The aim of this study was to screen for phytochemical constituents of T. coronaria in different solvent extraction in both qualitative and quantitative assays and to evaluate in vitro antioxidant activity inhibition of DPPH, FRAP, hydroxyl radical scavenging, superoxide radical scavenging, reducing power assay and nitric oxide radical scavenging activity. Results indicate that ethanolic extract of Tabernaemontana coronaria have marked high content of secondary metabolites among all the solvents. The total carbohydrates, phenol and flavanoid were 74.03mg/g, 98.08 mg/g, 24.28 mg/g. At (1003500µg/ml) concentration the T. coronaria of 500 µg/ml concentration exhibited high DPPH radical scavenging capacity using BHT as positive control. T. coronaria at the same concentration showed the dose dependent inhibition of FRAP, hydroxyl radical scavenging, superoxide radical scavenging, reducing power assay and nitric oxide radical scavenging activity. Collectively, our results indicate that the ethanolic extract of T.coronaria has the potential to scavenge free radicals and act as a good antioxidant for treating various diseases.