Free Radical Scavenging Activity and Total Phenolic content of Ziziphus Mauritiana Lam.

The free radical scavenging potential of the ethanolic and aqueous extracts of the leaves of Ziziphus mauritiana Lam, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation assay, superoxide scavenging activity, nitric oxide scavenging activity, total antioxidant capacity and Nonenzymatic Glycosylation of Hemoglobin assay. The results were analyzed tatistically by regression method. The percentage scavenging and IC50 values were calculated for all models. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The measurement of total phenolic content by Folin-Ciocalteus reagent indicated that ethanolic and aqueous extract contains total phenolic compound 0.122 mg/ml and 0.150 mg/ml equivalent to gallic acid. The results indicate that both ethanolic and aqueous extract of Z. mauritiana had significant antioxidant activity.