Inhibition of Transfected PTEN on Human Colon Cancer

Objective To study the inhibitory effect of transfected PTEN on LoVo cells. Methods The human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analysized by Western blot and flow cytometry. Before or after transfection, the activities of 5-Fu in inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. Results PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cell. Conclusion Transfected PTEN remarkably up-regulated PTEN expression in LoVo cells and promoted the apoptosis. PTEN transfection associated with 5-Fu treatment had cooperatively cytotoxic effect.