2016International journal of innovation and applied studiesRequires access

Identification and quantification of mycotoxins detected from different strains of fungi grown on PDA culture medium in the presence of eggs of Achatina fulica (Bowdich)

Dedi née Ky Juliette, Atcho Otchoumou, Allou Kouassi

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Abstract

The characterization of the fungal flora of the breeding litter Achatina fulica and different incubation substrates (coconut husks, cotton wool, sawdust, planting soil, virgin forest soil) of the eggs revealed the presence cosmopolitan fungi such as Aspergillus Niger, Fusarium oxysporum, Fusarium solani, Mucor sp., Penicillium decumbens, Penicillium sp. and Trichoderma sp. Note however that fusaria are only present on the breeding litter the giant African snail. The percentage of occurrence of fungi varies from one substrate to another. Aspergillus Niger remains the only fungus present on all substrates and Penicillium decumbens only on the planting soil. Similarly, the characterization of the potential of each of toxigenic strains performed through the identification technique by HPLC was able to detect and quantify ochratoxin A and aflatoxin G2, G1, B1 at most samples (eggs + fungus ) except that consisting of eggs + Fusarium solani or no mycotoxins were detected. Ochratoxin A detected in samples containing Aspergillus Niger, Oxysporum Fusarium, Mucor sp. and Penicillium sp. is present in very small quantities in the set. The aflatoxin B2 is absent in all samples.

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What this paper is about

The characterization of the fungal flora of the breeding litter Achatina fulica and different incubation substrates (coconut husks, cotton wool, sawdust, planting soil, virgin forest soil) of the eggs revealed the presence cosmopolitan fungi such as Aspergillus Niger, Fusarium oxysporum, Fusarium solani, Mucor sp., Penicillium decumbens, Penicillium sp. and Trichoderma sp. Note however that fusaria are only present on the breeding litter the giant African snail. The percentage of occurrence of fungi varies from one substrate to another. Aspergillus Niger remains the only fungus present on all substrates and Penicillium decumbens only on the planting soil. Similarly, the characterization of the potential of each of toxigenic strains performed through the identification technique by HPLC was able to detect and quantify ochratoxin A and aflatoxin G2, G1, B1 at most samples (eggs + fungus ) except that consisting of eggs + Fusarium solani or no mycotoxins were detected. Ochratoxin A detected in samples containing Aspergillus Niger, Oxysporum Fusarium, Mucor sp. and Penicillium sp. is present in very small quantities in the set. The aflatoxin B2 is absent in all samples.

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Available abstract

The characterization of the fungal flora of the breeding litter Achatina fulica and different incubation substrates (coconut husks, cotton wool, sawdust, planting soil, virgin forest soil) of the eggs revealed the presence cosmopolitan fungi such as Aspergillus Niger, Fusarium oxysporum, Fusarium solani, Mucor sp., Penicillium decumbens, Penicillium sp. and Trichoderma sp. Note however that fusaria are only present on the breeding litter the giant African snail. The percentage of occurrence of fungi varies from one substrate to another. Aspergillus Niger remains the only fungus present on all substrates and Penicillium decumbens only on the planting soil. Similarly, the characterization of the potential of each of toxigenic strains performed through the identification technique by HPLC was able to detect and quantify ochratoxin A and aflatoxin G2, G1, B1 at most samples (eggs + fungus ) except that consisting of eggs + Fusarium solani or no mycotoxins were detected. Ochratoxin A detected in samples containing Aspergillus Niger, Oxysporum Fusarium, Mucor sp. and Penicillium sp. is present in very small quantities in the set. The aflatoxin B2 is absent in all samples.

Key concepts: Biology, Penicillium, Fusarium solani, Fusarium oxysporum, Fusarium, Botany, Aspergillus flavus, Sterigmatocystin

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Identification and quantification of mycotoxins detected from different strains of fungi grown on PDA culture medium in the presence of eggs of Achatina fulica (Bowdich) — Research Paper | ScholarLens