Effect of Acidic and Basic Medium on Celecoxib by UV Spectroscopy

Degradation is a main problem in numerous unstable products. Our study on drug substance involves Acid/ base Stress testing. In our recent research we study the effect of different environmental conditions by subjecting the different brands of pharmaceutical formulation i.e. celecoxib (Nuzib, Seleco, Celbexx) under acidic and basic conditions by using spectrophotometer. It is usually preferred over other methods because of less equipment cost and economical maintenance advantage. When celecoxib (Nuzib, Seleco, and Celbexx) subjected to 0.1 N HCl and 0.1 N NaOH, celecoxib showed decreased availability in acidic medium as well as in alkaline medium. The absorbance in basic medium of different brands of celecoxib i.e., Nuzib, Seleco and Celbexx was found to be 0.055, 0.094, 0.071 and their percent availability was found to be 50%, 42.30%, 35.14%. In acidic medium the absorbance of Nuzib, Seleco and Celebex was found to be 0.047, 0.128, 0.074 percent availability was 42.70%, 57.60%, 36.63%. We conclude that the absorbance of all brands of celecoxib decreases in acidic and basic medium which is due to the degradation of celecoxib in both mediums.