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[Gene expression of D-beta-hydroxybutyrate dehydrogenase. III. Molecular cloning of a DNAc].

A. Bailly, A. Kante, Mustapha Cherkaoui‐Malki, Pascale Adami, Norbert Latruffe

Abstract

In order to continue the molecular studies of D-beta-hydroxybutyrate dehydrogenase (BDH) undertaken in our laboratory for several years, we have initiated a genetic approach which consists in the BDH cDNA cloning from a rat liver cDNA library. The immunoscreening method allowed to isolate a clone which exhibits a DNA insert shorter than the expected full length BDH cDNA.

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Key concepts: Complementary DNA, Cloning (programming), Immunoscreening, Molecular biology, Molecular cloning, cDNA library, Biology, Gene