1997•DNA sequenceRequires access

The Cloning and Sequencing of an Ovinec-myccDNA

Véronique Kiermer, Samuel Dequiedt, R Masengo, Y. Cleuter, Delphine Briclet, Malgorzata Ciesiolka, Anne Van den Broeke, Luc Willems, Richard Kettmann, Arsène Burny, Louis Droogmans

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Abstract

We report the cloning of an ovine c-myc cDNA. The clone was isolated from a bovine leukemia virus-infected cell line (YR2) cDNA library cloned in the lambda gt10 vector. The clone encodes the full length c-Myc protein made of 439 amino-acids with 93, 96, 92 and 93% similarity with human, feline, murine and rat c-Myc proteins, respectively.

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Key concepts: Complementary DNA, Cloning (programming), clone (Java method), Biology, cDNA library, Molecular biology, Molecular cloning, Vector (molecular biology)