Determination of flavonoid compounds in Gingko biloba leaves supercritical fluid extraction and high performance liquid chromatography (SFE/HPLC)
WX Yao, XM Cheng, Zhang YQ
Abstract
WX Yao, XM Cheng, Zhang YQ
Abstract
This paper discussed the determination method of flavonoid compounds in Gingko biloba leaves with supercritical CO2 and HPLC,The method is simple and accurate. It can eliminate the toxicity of residual solvents in organic solvent extracts. The average recovery is 87.3% (n=4), relative standard deviation (RSD) is 5.4% and the method detection limit (MDL) is 28ppb. The flavonoid content determined was 1.4%(w/w) in Gingko biloba leaves.
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This paper discussed the determination method of flavonoid compounds in Gingko biloba leaves with supercritical CO2 and HPLC,The method is simple and accurate. It can eliminate the toxicity of residual solvents in organic solvent extracts. The average recovery is 87.3% (n=4), relative standard deviation (RSD) is 5.4% and the method detection limit (MDL) is 28ppb. The flavonoid content determined was 1.4%(w/w) in Gingko biloba leaves.
Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Relative standard deviation, Flavonoid, Supercritical fluid, Supercritical fluid extraction, Detection limit