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[Inhibitory effect of isorhmnetin on telomerase activity of HeLa cells].

Chunlei Yang, Yi Qu, Zhengrong Wang, Dachang Tao

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Abstract

OBJECTIVE: To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells. METHODS: The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity. RESULTS: The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase. CONCLUSION: By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.

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What this paper is about

OBJECTIVE: To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells. METHODS: The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity. RESULTS: The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase. CONCLUSION: By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.

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Available abstract

OBJECTIVE: To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells. METHODS: The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity. RESULTS: The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase. CONCLUSION: By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.

Key concepts: HeLa, Telomerase, Apoptosis, Flow cytometry, In vitro, Molecular biology, Chemistry, Cell growth

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