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2003•Journal of Southeast UniversityRequires access

A study on the relationship between apoptosis of HeLa cells induced by As_2O_3 and inhibition of telomerase activity

Jie Zheng

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Abstract

Objective To study the inductive function of As 2O 3 on apoptosis of HeLa cells and its influence on telomerase activity.Methods HeLa cells were treated with As 2O 3 of various concentrations and observed at 24,48 and 72?h for morpho?logical alterations.The effect of As 2O 3 on HeLa cells was determined by MTT assay,and telomerase activity in HeLa cells was determined by TRAP?ELISA.Results 2?μmol·L -1As 2O 3 induced apoptosis of HeLa cells at 48?h and reduced their survival,and could also suppress telomerase activity in the cells under proper controls.It was found that the higher the As 2O 3 concentration was and the longer the treating time was,the better the effect was.Conclusion As 2O 3 inhibiting the telomerase activity in HeLa cells may be involved in the mechanism of the cell apoptosis.

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Objective To study the inductive function of As 2O 3 on apoptosis of HeLa cells and its influence on telomerase activity.Methods HeLa cells were treated with As 2O 3 of various concentrations and observed at 24,48 and 72?h for morpho?logical alterations.The effect of As 2O 3 on HeLa cells was determined by MTT assay,and telomerase activity in HeLa cells was determined by TRAP?ELISA.Results 2?μmol·L -1As 2O 3 induced apoptosis of HeLa cells at 48?h and reduced their survival,and could also suppress telomerase activity in the cells under proper controls.It was found that the higher the As 2O 3 concentration was and the longer the treating time was,the better the effect was.Conclusion As 2O 3 inhibiting the telomerase activity in HeLa cells may be involved in the mechanism of the cell apoptosis.

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Available abstract

Objective To study the inductive function of As 2O 3 on apoptosis of HeLa cells and its influence on telomerase activity.Methods HeLa cells were treated with As 2O 3 of various concentrations and observed at 24,48 and 72?h for morpho?logical alterations.The effect of As 2O 3 on HeLa cells was determined by MTT assay,and telomerase activity in HeLa cells was determined by TRAP?ELISA.Results 2?μmol·L -1As 2O 3 induced apoptosis of HeLa cells at 48?h and reduced their survival,and could also suppress telomerase activity in the cells under proper controls.It was found that the higher the As 2O 3 concentration was and the longer the treating time was,the better the effect was.Conclusion As 2O 3 inhibiting the telomerase activity in HeLa cells may be involved in the mechanism of the cell apoptosis.

Key concepts: HeLa, Telomerase, Apoptosis, Molecular biology, MTT assay, Chemistry, Telomere, Cell

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