[Effects on DNA damage and apoptosis and p53 protein expression induced by fluoride in human embryo hepatocytes].

OBJECTIVE: To study the effects of DNA damage and apoptosis and p53 expression, and to explore the relationship between apoptosis and p53 expression in human embryo hepatocytes induced by fluoride. METHODS: The rate of DNA damage, apoptosis and the level of p53 expression were investigated after the cells were incubated with sodium fluoride for about 24 hours. The concentrations of sodium fluoride of the control, A, B and C group were 0 microg/ml, 40 microg/ ml, 80 microg/ml and 160 microg/ml individually. RESULTS: The rate of DNA damage of every group treated with sodium fluoride were significantly higher than the control group (P < 0.05). The percentage of apoptosis of B and C groups increased apparently (P < 0.05). The level of p53 expression of B and C groups were significantly higher than the control group (P < 0.01). CONCLUSION: Fluoride can increase the rate of DNA damage, and induce apoptosis and expression of p53 in human embryo hepatocytes. Furthermore, both apoptosis and the level of p53 expression, there exists a rise tendency with the increase concentration of fluoride.