1988•Annals of PharmacotherapyRequires access

Marked Digoxin-Likeimmunoreactive Factor Interference with an Enzyme Immunoassay

James A. Karboski, Paul J. Godley, Paul Frohna, Michael W. Horton, William J. Reitmeyer

Abstract

A case in which digoxin-like immunoreactive factors (DLIF) interfered with an enzyme immunoassay in a patient with renal insufficiency is reported. A 79-year-old woman was found to have a serum digoxin concentration (SDC) determined by enzyme immunoassay of 5.0 ng/ml. Although all subsequent SDC determined by the enzyme immunoassay system were elevated, identical samples run on a fluorescence polarization immunoassay revealed SDC within the therapeutic range. Marked DLIF-related assay interference has been reported to occur with some digoxin assays; however, the enzyme immunoassay methods have never been reported to cross-react to the magnitude seen in this case.

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Key concepts: Immunoassay, Digoxin, Fluorescence polarization immunoassay, Enzyme, Chemistry, Radioimmunoassay, Chromatography, Medicine