ScholarLens Back to search
2016•Research Journal of Pharmacy and TechnologyOpen access

Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Esomeprazole and Domperidone in Tablet Formulation

Vitthal S. Hiwale, Sagar M. Bhangale, Sachin S. Rane, Milind E. Chaudhari, Rajesh Y. Chaudhari, Vijay R. Patil

Open full text 0 citations

Abstract

A new simple, rapid, precise, and specific assay method was developed for simultaneous estimation of Esomeprazole and Domperidone in pure form and tablet form. The analysts were separated by RP HPLC on a RP-Purosnosphere C18 column (5 μm, 4.6mm, 250 mm). The mobile phase was methanol: water (72: 28 v/v) at 1.0 mL/min satisfactorily resolved the binary mixture. The UV detector was operated at 289 nm for the determination of both the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 20–120 μg/ml for Esomeprazole and Domperidone with a R2 0.9998 and 0.9990 values respectively, in binary mixture. The optimized method proved to be specific, robust and accurate for the quality control in bulk drug and pharmaceutical formulations.

About this research paper

What this paper is about

A new simple, rapid, precise, and specific assay method was developed for simultaneous estimation of Esomeprazole and Domperidone in pure form and tablet form. The analysts were separated by RP HPLC on a RP-Purosnosphere C18 column (5 μm, 4.6mm, 250 mm). The mobile phase was methanol: water (72: 28 v/v) at 1.0 mL/min satisfactorily resolved the binary mixture. The UV detector was operated at 289 nm for the determination of both the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 20–120 μg/ml for Esomeprazole and Domperidone with a R2 0.9998 and 0.9990 values respectively, in binary mixture. The optimized method proved to be specific, robust and accurate for the quality control in bulk drug and pharmaceutical formulations.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A new simple, rapid, precise, and specific assay method was developed for simultaneous estimation of Esomeprazole and Domperidone in pure form and tablet form. The analysts were separated by RP HPLC on a RP-Purosnosphere C18 column (5 μm, 4.6mm, 250 mm). The mobile phase was methanol: water (72: 28 v/v) at 1.0 mL/min satisfactorily resolved the binary mixture. The UV detector was operated at 289 nm for the determination of both the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 20–120 μg/ml for Esomeprazole and Domperidone with a R2 0.9998 and 0.9990 values respectively, in binary mixture. The optimized method proved to be specific, robust and accurate for the quality control in bulk drug and pharmaceutical formulations.

Key concepts: Domperidone, Esomeprazole, Mathematics, Chromatography, Biomedical engineering, Computer science, Medicine, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Esomeprazole and Domperidone in Tablet Formulation — Research Paper | ScholarLens