Northern blot analysis of total RNA extracted from primary cultures of SECs and MNPs at day 1 after isolation either untreated (c) or treated with IFN-γ 10000 (10)U/ml (I) or IFN-γ together with TNF-β (10 ng/ml) (I T) for 12 h

Five μg of total RNA was separated on agarose gel, blotted and hybridised with a P-dCTP-labeled cDNA probe specific for rat PECAM-1. The same membranes were hybridised with a cDNA specific for rat ICAM-1 or for β- and γ-actin. This figure shows results of five experiments from five different isolations. The lower panel shows a statistical evaluation of densitometric scans. Error bars indicate standard deviation of the mean value after normalization on β,γ-actin expression. P-value was tested to show significant differences of control cells compared to cells treated with IFN-γ or IFN-γ +TNF-β. * indicates p < 0.05.Copyright information:Taken from "Decrease of PECAM-1-gene-expression induced by proinflammatory cytokines IFN-γ and IFN-α is reversed by TGF-β in sinusoidal endothelial cells and hepatic mononuclear phagocytes"http://www.biomedcentral.com/1472-6793/8/9BMC Physiology 2008;8():9-9.Published online 8 May 2008PMCID:PMC2396664.