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2011•Journal of Medical ForumRequires access

Effect of IFN-γ on B7-H1 expression of leukemia cell line U937

Guo Jian-min

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Abstract

Objective To investigate the effect of interferon-γ(IFN-γ) on B7-H1 expression of leukemia cell line U937.Methods After being treated with different doses of IFN-γ for 48 hours,B7-H1 expression of U937 cell was detected by immunohistochemistry,flow cytometry and real-time quantitative RT-PCR.Results Compared with that of control group(IFN-γ,0ng/ml),the levels of protein expression of B7-H1 were significantly higher after being treated by IFN-γ(50ng/ml,100ng/ml and 150ng/ml)(P0.001) in dose dependent manner.IFN-γ could up-regulate the mRNA expression of B7-H1 on U937 in dose dependent manner.The B7-H1 gene expression of IFN-γ(50ng/ml、100ng/ml and 150ng/ml) groups were 6.5 times,26 times and 22.7 times higher than that of control group respectively.Conclusions IFN-γ could remarkably up-regulated the B7-H1 expressions of leukemia cell line U937 in dose dependent manner.

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Objective To investigate the effect of interferon-γ(IFN-γ) on B7-H1 expression of leukemia cell line U937.Methods After being treated with different doses of IFN-γ for 48 hours,B7-H1 expression of U937 cell was detected by immunohistochemistry,flow cytometry and real-time quantitative RT-PCR.Results Compared with that of control group(IFN-γ,0ng/ml),the levels of protein expression of B7-H1 were significantly higher after being treated by IFN-γ(50ng/ml,100ng/ml and 150ng/ml)(P0.001) in dose dependent manner.IFN-γ could up-regulate the mRNA expression of B7-H1 on U937 in dose dependent manner.The B7-H1 gene expression of IFN-γ(50ng/ml、100ng/ml and 150ng/ml) groups were 6.5 times,26 times and 22.7 times higher than that of control group respectively.Conclusions IFN-γ could remarkably up-regulated the B7-H1 expressions of leukemia cell line U937 in dose dependent manner.

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Available abstract

Objective To investigate the effect of interferon-γ(IFN-γ) on B7-H1 expression of leukemia cell line U937.Methods After being treated with different doses of IFN-γ for 48 hours,B7-H1 expression of U937 cell was detected by immunohistochemistry,flow cytometry and real-time quantitative RT-PCR.Results Compared with that of control group(IFN-γ,0ng/ml),the levels of protein expression of B7-H1 were significantly higher after being treated by IFN-γ(50ng/ml,100ng/ml and 150ng/ml)(P0.001) in dose dependent manner.IFN-γ could up-regulate the mRNA expression of B7-H1 on U937 in dose dependent manner.The B7-H1 gene expression of IFN-γ(50ng/ml、100ng/ml and 150ng/ml) groups were 6.5 times,26 times and 22.7 times higher than that of control group respectively.Conclusions IFN-γ could remarkably up-regulated the B7-H1 expressions of leukemia cell line U937 in dose dependent manner.

Key concepts: Flow cytometry, U937 cell, Leukemia, Interferon, Cell culture, Cell, Immunohistochemistry, Molecular biology

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