2015•PubMedRequires access
[Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells].
Chao Fan, Mingyu Fu, Zhongju Xiao, Jie Tang
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Abstract
OBJECTIVE: To express solute carrier 26A proteins in HEK-293 cells and explore their functions. METHODS: SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording. RESULTS: All the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions. CONCLUSION: The SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.