2006•PubMedRequires access
[Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer].
Xiaoqu Hu, Li Qin, Fengxi Su, Herui Yao, Jisheng Chen, Chang Gong, Ju-jiang Guo, Fengyan Yu, Haixia Jia
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Abstract
OBJECTIVE: To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy. METHODS: A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection. RESULTS: The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells. CONCLUSION: HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.