Inhibition of cell proliferation of lung cancer by vector based-small interfering RNA targeting HER2 gene

Objective To construct RNA interference vector to down-regulate HER2 gene and to study the RNA interference effect on the cell cycle and tumor growth of non-small-cell lung cancer (NSCLC).Methods Oligonucleotides of 64 base pairs for hairpin RNA targeting HER2,designed,chemi- cally synthesized,and annealed,were cloned into pcDNA3 vector.After identification by restriction diges- tion,the right vectors were transiently transfected into SPC-A-1 cell,a human lung adenocarcinoma cell line.HER.2 mRNA and HER2 protein were detected by RT-PCR,and Western Blot,respectively.FCM anal- ysis and MTr method were applied to measure cell cycle and cell growth.Results The vector of RNA inter- ference which could interfere HER2 gene was successfully constructed,which could increase the number of cells in G_0/G_1 phase by 9.9% and inhibit the tumor cell growth.Conclusion We have successfully con- structed expressing hairpin RNA against HER2 vector.The vector can effectively silence HER2 gene,in- crease the number of cells in G_0/G_1 phase,and then slow down the growth of tumor cells.This may be a use- ful therapeutic strategy for HER2 over-expressing NSCLC.