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[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].

Manli Gong, Ren Nan-qi, Defeng Xing

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Abstract

In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.

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What this paper is about

In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.

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OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.

Key concepts: Temperature gradient gel electrophoresis, Gel electrophoresis, Electrophoresis, Pulsed-field gel electrophoresis, Two-dimensional gel electrophoresis, Microbial ecology, Biology, Chromatography

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[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology]. — Research Paper | ScholarLens