1998•Current Protocols in Human GeneticsRequires access

Detection of Mutations by Denaturing Gradient Gel Electrophoresis

Anne‐Lise Børresen‐Dale, Eivind Hovig, Birgitte Smith‐Sørensen

Abstract

This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.

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Available abstract

Key concepts: Temperature gradient gel electrophoresis, Gel electrophoresis, Electrophoresis, Chromatography, Gel electrophoresis of nucleic acids, Perpendicular, Gel electrophoresis of proteins, Chemistry