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2016•Indian Journal of Plant Genetic ResourcesOpen access

Molecular Characterization of Cabbage (Brassica oleraceaL. var.capitata) Genotypes using RAPD Markers

Nisha Thakur, Dhiraj Kumar Srivastava

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Abstract

The genetic relationship among sixteen genotypes of cabbage were studied using RAPD markers. Cabbage genotypes were collected from IARI Research Centre, Kattarine, Kullu and Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan. A total of 17 primers were used to generate RAPD profiles, out of these 7 primers gave reproducible banding patterns. A total of 22 bands were obtained and all were polymorphic with two cultivar specific bands. The percentage of primer polymorphism was 100%. Similarity index was computed based on Jaccard's similarity coefficient and used for cluster analysis based on UPGMA. At 62% similarity level sixteen genotypes were grouped into two clusters. RAPD technology could be useful for identification of different genotypes as-well-as accessing the genetic similarity among different accessions of cabbage.

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The genetic relationship among sixteen genotypes of cabbage were studied using RAPD markers. Cabbage genotypes were collected from IARI Research Centre, Kattarine, Kullu and Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan. A total of 17 primers were used to generate RAPD profiles, out of these 7 primers gave reproducible banding patterns. A total of 22 bands were obtained and all were polymorphic with two cultivar specific bands. The percentage of primer polymorphism was 100%. Similarity index was computed based on Jaccard's similarity coefficient and used for cluster analysis based on UPGMA. At 62% similarity level sixteen genotypes were grouped into two clusters. RAPD technology could be useful for identification of different genotypes as-well-as accessing the genetic similarity among different accessions of cabbage.

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Available abstract

The genetic relationship among sixteen genotypes of cabbage were studied using RAPD markers. Cabbage genotypes were collected from IARI Research Centre, Kattarine, Kullu and Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan. A total of 17 primers were used to generate RAPD profiles, out of these 7 primers gave reproducible banding patterns. A total of 22 bands were obtained and all were polymorphic with two cultivar specific bands. The percentage of primer polymorphism was 100%. Similarity index was computed based on Jaccard's similarity coefficient and used for cluster analysis based on UPGMA. At 62% similarity level sixteen genotypes were grouped into two clusters. RAPD technology could be useful for identification of different genotypes as-well-as accessing the genetic similarity among different accessions of cabbage.

Key concepts: RAPD, UPGMA, Jaccard index, Biology, Genotype, Forensic science, Similarity (geometry), Veterinary medicine

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Molecular Characterization of Cabbage (Brassica oleraceaL. var.capitata) Genotypes using RAPD Markers — Research Paper | ScholarLens