2001•PubMedRequires access
Expression of the Recombinant Human Interleukin-11 in Pichia pastoris.
Wang Ty, Wang Cm, Guoqing Wei, JW Qiu, Huang Ys
Open publisher page 4 citations
Abstract
Full length cDNA of human IL-11 was synthesized by DNA synthesizer. An expression plasmid, pGENYk, containing the recombinant DNA fragment, was linearized and transformed into Pichia pastoris. This recombinant gene was highly expressed in this yeast, and the expression product was purified by a three-step chromatography method. Analysis of the purified recombination protein with SDS-PAGE, Western blot and biological activities showed that the activity of the protein was the same as the Neumega expressed in E.coli.