ScholarLens Back to search
2004•Unpublished venueRequires access

Expression and characterization of vWF binding domain of platelet GPIbα in Pichia pastoris

Yu Zhang

Open publisher page 0 citations

Abstract

Objective: To express vWF binding domain of platelet GPⅠbα in Pichia pastoris. Methods: The gene of vWF binding domain of platelet GPⅠbα was obtained by polymerase chain reaction(PCR) from recombinant plasmid pcDNA3.1-GP Ⅰbα.The recombinant expression vector was constructed and was electroporated into pichia pastoris GS115. The transformants were screened by G418 and induced by methanol. The expressed protein was identified by SDS-PAGE and dot blot. Results: Recombinant plasmid pPIC9k-GP302 was obtained.Positive colonies were obtained by G418 screening and were induced by methanol.The expressed protein had ability to bind anti-GPⅠbα monoclonal antibody, which was identified by dot blot. Conclusion: vWF binding domain of platelet GPⅠbα was expressed in Pichia pastoris.

About this research paper

What this paper is about

Objective: To express vWF binding domain of platelet GPⅠbα in Pichia pastoris. Methods: The gene of vWF binding domain of platelet GPⅠbα was obtained by polymerase chain reaction(PCR) from recombinant plasmid pcDNA3.1-GP Ⅰbα.The recombinant expression vector was constructed and was electroporated into pichia pastoris GS115. The transformants were screened by G418 and induced by methanol. The expressed protein was identified by SDS-PAGE and dot blot. Results: Recombinant plasmid pPIC9k-GP302 was obtained.Positive colonies were obtained by G418 screening and were induced by methanol.The expressed protein had ability to bind anti-GPⅠbα monoclonal antibody, which was identified by dot blot. Conclusion: vWF binding domain of platelet GPⅠbα was expressed in Pichia pastoris.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective: To express vWF binding domain of platelet GPⅠbα in Pichia pastoris. Methods: The gene of vWF binding domain of platelet GPⅠbα was obtained by polymerase chain reaction(PCR) from recombinant plasmid pcDNA3.1-GP Ⅰbα.The recombinant expression vector was constructed and was electroporated into pichia pastoris GS115. The transformants were screened by G418 and induced by methanol. The expressed protein was identified by SDS-PAGE and dot blot. Results: Recombinant plasmid pPIC9k-GP302 was obtained.Positive colonies were obtained by G418 screening and were induced by methanol.The expressed protein had ability to bind anti-GPⅠbα monoclonal antibody, which was identified by dot blot. Conclusion: vWF binding domain of platelet GPⅠbα was expressed in Pichia pastoris.

Key concepts: Pichia pastoris, Recombinant DNA, Pichia, Molecular biology, Expression vector, Western blot, Plasmid, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Expression and characterization of vWF binding domain of platelet GPIbα in Pichia pastoris — Research Paper | ScholarLens