Effect of Sulfur-containing Compounds on Experimental Diabetes. V. : Effect of Thiol and Its Oxidized Type Compounds on the Liberation of Panaeatic Insulin

Effect of SH and S-S compounds on the liberation of insulin from rat pancreas was examined. (1) Addition of SH or S-S compounds does not affect liberation when glucose was given in an amount of 2 mg/ml of the reaction medium, but with 4 mg/ml of glucose, SH compounds accelerate the liberation and S-S compounds do not. The same tendency is observed in the liberation of insulin from the pancreas of a rat preliminarily given SH or S-S compounds. (2) p-Chloromercuribenzoate (PCMB) interferes with liberation and this interference does not disappear even by addition of SH compounds after pretreatment with PCMB. (3) Addition of insulin to the reaction solution inhibits the liberation. This fact suggests that liberation of insulin from the pancreas is regulated not only by glucose but also by insulin.