1995•PubMedRequires access
Optimal preservation of the J1-31 antigen in astroglia examined by immunogold labelling.
Rakesh Kumar Bhatnagar, SK Malhotra
Open publisher page 1 citations
Abstract
Several methods for the detection of immunogold labelling for the monoclonal antibody (Mab) J1-31 in astroglial cells are described. The optimal preservation of the gold labelling is achieved in sections after cryoultramicrotomy or LR white embedding. The results obtained are consistent with the previous findings that the Mab J1-31 antigen is an intermediate filament-associated protein in the astroglial cells.